Mouse IgM(Immunoglobulin M) ELISA Kit

2h

IgM

54ng/mL

100000ng/mL

Mus musculus

160-100000ng/mL

Competitive Inhibition

Infection immunity;Immune molecule;Hematology;

IGHM; Immunoglobulin Heavy Constant Mu; Ig mu chain C region

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Immunoglobulin M (IgM) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Immunoglobulin M (IgM) and unlabeled Immunoglobulin M (IgM) (Standards or samples) with the pre-coated antibody specific to Immunoglobulin M (IgM). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Immunoglobulin M (IgM) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Immunoglobulin M (IgM) in the sample.