Rat MEP1a(Meprin A Alpha) ELISA Kit

2h

4000pg/mL

15.2pg/mL

49.4-4000pg/mL

Rattus norvegicus

Metabolic pathway;

Competitive Inhibition

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

The MEP1a(Meprin A Alpha) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

PPHA; PABA Peptide Hydrolase; Endopeptidase-2; N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Alpha; PABA Peptide Hydrolase

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Meprin A Alpha (MEP1α) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Meprin A Alpha (MEP1α) and unlabeled Meprin A Alpha (MEP1α) (Standards or samples) with the pre-coated antibody specific to Meprin A Alpha (MEP1α). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Meprin A Alpha (MEP1α) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Meprin A Alpha (MEP1α) in the sample.