Rabbit NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

2h

brain

47.2pg/mL

10000pg/mL

123.5-10000pg/mL

NT-Pro-BNP; ; N-BNP

Oryctolagus cuniculus

Competitive Inhibition

Endocrinology;Cardiovascular biology;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Rabbits are used for polyclonal antibody production by ELK Biotech. Rabbit antibodies are very stable and can be stored for several days at room temperature. ELK Biotech adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) and unlabeled N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) (Standards or samples) with the pre-coated antibody specific to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) in the sample.