Mouse SALb(Salusin Beta) ELISA Kit

 

Mouse SALb(Salusin Beta) ELISA Kit

Size

96T

Catalog no.

ELK5543

Price

608 EUR

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Assay length

2h

Standard

400pg/mL

Sensitivity

1.82pg/mL

Latin name

Mus musculus

Detection range

4.94-400pg/mL

Assay Type

Competitive Inhibition

Research Area

Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Salusin Beta (SALβ) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Salusin Beta (SALβ) and unlabeled Salusin Beta (SALβ) (Standards or samples) with the pre-coated antibody specific to Salusin Beta (SALβ). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Salusin Beta (SALβ) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Salusin Beta (SALβ) in the sample.