Human VF(Visfatin) ELISA Kit

 

Human VF(Visfatin) ELISA Kit

Size

96T

Catalog no.

ELK5561

Price

608 EUR

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Assay length

2h

Standard

100ng/mL

Sensitivity

0.51ng/mL

Detection range

1.23-100ng/mL

Assay Type

Competitive Inhibition

Research Area

Metabolic pathway;Cardiovascular biology;Hepatology;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Alternative Names

Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Visfatin (VF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Visfatin (VF) and unlabeled Visfatin (VF) (Standards or samples) with the pre-coated antibody specific to Visfatin (VF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Visfatin (VF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Visfatin (VF) in the sample.