Human TACTILE(T-Cell Activation Antigen, Increased Late Expression) ELISA Kit

 

Human TACTILE(T-Cell Activation Antigen, Increased Late Expression) ELISA Kit

Size

96T

Catalog no.

ELK5595

Price

608 EUR

Buy at gentaur.com
Assay length

3h

Tissue

cell

Sensitivity

29pg/mL

Assay Type

Sandwich

Standard

5000pg/mL,

Detection range

78-5000pg/mL,

Research Area

CD & Adhesion molecule;

Alternative Names

CD96; T-cell surface protein tactile

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Description

Antigens are peptides or recombinant or native dependent on the production method.For cells, cell lines and tissues in culture till half confluency.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Cell Activation Antigen, Increased Late Expression (TACTILE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to T-Cell Activation Antigen, Increased Late Expression (TACTILE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Cell Activation Antigen, Increased Late Expression (TACTILE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T-Cell Activation Antigen, Increased Late Expression (TACTILE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.