3h
96T
ELK5629
738 EUR
3h
24pg/mL
Sandwich
5000pg/mL
78.12-5000pg/mL
Oryctolagus cuniculus
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Signal transduction;Metabolic pathway;Apoptosis;Endocrinology;Cardiovascular biology;Neuro science;
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
OLR1; CLEC8A; SCARE1; Oxidized low-density lipoprotein receptor 1; C-type lectin domain family 8 member A; Lectin-like oxidized LDL receptor 1; Lectin-type oxidized LDL receptor 1
Rabbits are used for polyclonal antibody production by ELK Biotech. Rabbit antibodies are very stable and can be stored for several days at room temperature. ELK Biotech adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
High and low density proteins are supplied by ELK Biotech in volumes of 1. Other densities are possible.The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.