Pig IL1a(Interleukin 1 Alpha) ELISA Kit

 

Pig IL1a(Interleukin 1 Alpha) ELISA Kit

Size

96T

Catalog no.

ELK5644

Price

738 EUR

Buy at gentaur.com
Assay length

3h

Sensitivity

6.6pg/mL

Assay Type

Sandwich

Standard

1000pg/mL

Detection range

15.6-1000pg/mL

Research Area

Cytokine;Apoptosis;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

About

Pigs and the smaller guinea pigs are frequent used as models for humans.

Description

The IL1a(Interleukin 1 Alpha) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Alternative Names

IL1 Alpha,IL1-A; IL-1α; IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; Interleukin-1 Family Member 1

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Alpha (IL1α). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Alpha (IL1α). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Alpha (IL1α), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Alpha (IL1α) in the samples is then determined by comparing the O.D. of the samples to the standard curve.