Rat PKCz(Protein Kinase C Zeta) ELISA Kit

 

Rat PKCz(Protein Kinase C Zeta) ELISA Kit

Size

96T

Catalog no.

ELK5751

Price

608 EUR

Buy at gentaur.com
Assay length

3h

Standard

100ng/mL

Assay Type

Sandwich

Sensitivity

0.65ng/mL

Detection range

1.56-100ng/mL

Latin name

Rattus norvegicus

Research Area

Signal transduction;Enzyme & Kinase;Neuro science;

Alternative Names

PKC Zeta,PKC2; PKC-Z; PRKCZ; Protein Kinase M Zeta

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase C Zeta (PKCζ). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase C Zeta (PKCζ). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase C Zeta (PKCζ), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase C Zeta (PKCζ) in the samples is then determined by comparing the O.D. of the samples to the standard curve.