Cattle HSPB1(Heat Shock 27kDa Protein 1) ELISA Kit

3h

Sandwich

2000pg/mL

13.2pg/mL

31.2-2000pg/mL

Signal transduction;Tumor immunity;Developmental science;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

HSP27; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock Protein 27; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 27kDa Protein 1 (HSPB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 27kDa Protein 1 (HSPB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 27kDa Protein 1 (HSPB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 27kDa Protein 1 (HSPB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.