Rat CCK8(Cholecystokinin 8, Octapeptide) ELISA Kit

2h

1000pg/mL

5.34pg/mL

Sincalide

12.35-1000pg/mL

Rattus norvegicus

Competitive Inhibition

Endocrinology;Gastroenterology;Hormone metabolism;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cholecystokinin 8, Octapeptide (CCK8) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cholecystokinin 8, Octapeptide (CCK8) and unlabeled Cholecystokinin 8, Octapeptide (CCK8) (Standards or samples) with the pre-coated antibody specific to Cholecystokinin 8, Octapeptide (CCK8). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cholecystokinin 8, Octapeptide (CCK8) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cholecystokinin 8, Octapeptide (CCK8) in the sample.