Cattle MMP3(Matrix Metalloproteinase 3) ELISA Kit

 

Cattle MMP3(Matrix Metalloproteinase 3) ELISA Kit

Size

96T

Catalog no.

ELK5808

Price

738 EUR

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Assay length

3h

Standard

10ng/mL

Assay Type

Sandwich

Sensitivity

0.064ng/mL

Detection range

0.156-10ng/mL

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Alternative Names

SL1; STMY; STMY1; STR1; Progelatinase; Stromelysin 1; Transin-1

Research Area

Enzyme & Kinase;Tumor immunity;Infection immunity;Cardiovascular biology;

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 3 (MMP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 3 (MMP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 3 (MMP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 3 (MMP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.