3h
Cattle CCKAR(Cholecystokinin A Receptor) ELISA Kit
Cattle CCKAR(Cholecystokinin A Receptor) ELISA Kit
Assay length
Standard
20ng/mL
Assay Type
Sandwich
Sensitivity
0.119ng/mL
Detection range
0.312-20ng/mL
Alternative Names
CCK-A; CCK1; CCKRA; CCK1R; Cholecystokinin Type 1 Receptor
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Research Area
Signal transduction;Endocrinology;Hepatology;Hormone metabolism;
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Description
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholecystokinin A Receptor (CCKAR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholecystokinin A Receptor (CCKAR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholecystokinin A Receptor (CCKAR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholecystokinin A Receptor (CCKAR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.