Cattle IgA(Immunoglobulin A) ELISA Kit

 

Cattle IgA(Immunoglobulin A) ELISA Kit

Size

96T

Catalog no.

ELK5827

Price

738 EUR

Buy at gentaur.com
Assay length

3h

Additional isotype

IgA

Standard

50ng/mL

Assay Type

Sandwich

Sensitivity

0.34ng/mL

Detection range

0.78-50ng/mL

Research Area

Infection immunity;Immune molecule;Hematology;Pulmonology;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a critical role in immune function in the mucous membranes. More IgA is produced in mucosal linings than all other types of antibody combined. Examples are EMA, ACA ELISA test kits and for diagnostic detection the IgA of Anti-Beta-2 Glycoprotein 1; β2-Glycoprotein 1 Antibodies; Beta 2GP1 Ab, Beta-2 Glycoprotein 1 Antibodies IgG, IgM, and IgAs.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin A (IgA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin A (IgA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin A (IgA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin A (IgA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.