Cattle TIMP2(Tissue Inhibitors Of Metalloproteinase 2) ELISA Kit

6

3h

tissue

Sandwich

4000pg/mL

26.5pg/mL

Tumor immunity;

62.50-4000pg/mL

CSC-21K; TIMP Metallopeptidase Inhibitor 2

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tissue Inhibitors Of Metalloproteinase 2 (TIMP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tissue Inhibitors Of Metalloproteinase 2 (TIMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tissue Inhibitors Of Metalloproteinase 2 (TIMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.