Cattle COL1a2(Collagen Type I Alpha 2) ELISA Kit

3h

400ng/mL

Sandwich

2.52ng/mL

6.25-400ng/mL

Metabolic pathway;Bone metabolism;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )

The Cattle COL1a2(Collagen I Alpha 2) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

COL1-A2; OI4; Collagen Alpha-2(I)chain; osteogenesis imperfecta type IV; Collagen Of Skin,Tendon And bone,Alpha-2 Chain

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type I Alpha 2 (COL1α2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type I Alpha 2 (COL1α2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type I Alpha 2 (COL1α2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type I Alpha 2 (COL1α2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.