3h
Dog aFP(Alpha-Fetoprotein) ELISA Kit
Dog aFP(Alpha-Fetoprotein) ELISA Kit
Assay length
Standard
20ng/mL
Assay Type
Sandwich
Sensitivity
0.137ng/mL
Detection range
0.312-20ng/mL
Research Area
Tumor immunity;Hepatology;
Alternative Names
A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Description
The aFP(Alpha-Fetoprotein) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.
Properties
Canine or Canis Lupus is mostly Beagle used for drug research. ELK Biotech produces ELISA test kits and polyclonal antibodies.E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Fetoprotein (αFP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Fetoprotein (αFP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Fetoprotein (αFP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-Fetoprotein (αFP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.