2h
96T
ELK5896
738 EUR
2h
1000pg/mL
4.85pg/mL
12.35-1000pg/mL
Competitive Inhibition
Endocrinology;Hormone metabolism;
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Pigs and the smaller guinea pigs are frequent used as models for humans.
AM; PAMP; Proadrenomedullin N-20 terminal peptide; ProAM N-terminal 20 peptide
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Adrenomedullin (ADM) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Adrenomedullin (ADM) and unlabeled Adrenomedullin (ADM) (Standards or samples) with the pre-coated antibody specific to Adrenomedullin (ADM). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adrenomedullin (ADM) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Adrenomedullin (ADM) in the sample.