Cattle aHSG(Alpha-2-Heremans Schmid Glycoprotein) ELISA Kit

3h

400ng/mL

Sandwich

2.45ng/mL

6.25-400ng/mL

Infection immunity;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

HSGa; A2HS; AHS; FETUA; HSG-A2; Fetuin-A; Alpha 2-HS Glycoprotein; Fetuin A; Alpha-2-Z-Globulin; Ba-alpha-2-Glycoprotein

The Cattle aHSG(Alpha-2-Heremans Schmid Glycoprotein) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Heremans Schmid Glycoprotein (αHSG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-2-Heremans Schmid Glycoprotein (αHSG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-2-Heremans Schmid Glycoprotein (αHSG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-2-Heremans Schmid Glycoprotein (αHSG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.