Cattle MCP2(Monocyte Chemotactic Protein 2) ELISA Kit

3h

6.4pg/mL

Sandwich

1000pg/mL

15.62-1000pg/mL

Cytokine;Infection immunity;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

CCL8; CCL-8; SCYA8; MCP-2; HC14; Chemokine C-C-Motif Ligand 8; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 8; Monocyte Chemoattractant Protein 2

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monocyte Chemotactic Protein 2 (MCP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Monocyte Chemotactic Protein 2 (MCP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Monocyte Chemotactic Protein 2 (MCP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Monocyte Chemotactic Protein 2 (MCP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.