Cattle MDC(Macrophage Derived Chemokine) ELISA Kit

3h

5.4pg/mL

Sandwich

1000pg/mL

15.62-1000pg/mL

Cytokine;Infection immunity;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Chemokines, chemokine receptors, ligands , motif chemokines and cytokines are supplied by ELK Biotech in 1.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

CCL22; ABCD1; DC/B-CK; SCYA22; STCP1; Chemokine C-C-Motif Ligand 22; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 22; Stimulated T-Cell Chemotactic Protein 1

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Derived Chemokine (MDC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Derived Chemokine (MDC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Derived Chemokine (MDC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Derived Chemokine (MDC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.