3h
Pig CTSK(Cathepsin K) ELISA Kit
Pig CTSK(Cathepsin K) ELISA Kit
Assay length
Standard
20ng/mL
Assay Type
Sandwich
Sensitivity
0.112ng/mL
Detection range
0.312-20ng/mL
Research Area
Enzyme & Kinase;Infection immunity;Bone metabolism;
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About
Pigs and the smaller guinea pigs are frequent used as models for humans.
Alternative Names
CTS-K; CTS02; CTSO; CTSO1; CTSO2; PKND; PYCD; Pycnodysostosis; Cathepsin O; Cathepsin X
Gene
CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathepsin K (CTSK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathepsin K (CTSK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathepsin K (CTSK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cathepsin K (CTSK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.