Dog MIP3b(Macrophage Inflammatory Protein 3 Beta) ELISA Kit

3h

5.6pg/mL

Sandwich

1000pg/mL

15.62-1000pg/mL

Cytokine;Infection immunity;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

CCL19; CKb11; ELC; MIP3-B; SCYA19; Chemokine C-C-Motif Ligand 19; Beta Chemokine Exodus-3; CK Beta-11; EBI1-Ligand Chemokine; Small Inducible Cytokine Subfamily A19

Canine or Canis Lupus is mostly Beagle used for drug research. ELK Biotech produces ELISA test kits and polyclonal antibodies.E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 3 Beta (MIP3β). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 3 Beta (MIP3β). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 3 Beta (MIP3β), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Inflammatory Protein 3 Beta (MIP3β) in the samples is then determined by comparing the O.D. of the samples to the standard curve.