Pig MMP13(Matrix Metalloproteinase 13) ELISA Kit

 

Pig MMP13(Matrix Metalloproteinase 13) ELISA Kit

Size

96T

Catalog no.

ELK5944

Price

738 EUR

Buy at gentaur.com
Assay length

3h

Assay Type

Sandwich

Standard

4000pg/mL

Sensitivity

28.1pg/mL

Detection range

62.50-4000pg/mL

Alternative Names

CLG3; Collagenase 3

Research Area

Enzyme & Kinase;Tumor immunity;Bone metabolism;Rheumatology;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

About

Pigs and the smaller guinea pigs are frequent used as models for humans.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 13 (MMP13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 13 (MMP13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 13 (MMP13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 13 (MMP13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.