Cattle GDH(Glutamate Dehydrogenase 1) ELISA Kit

3h

500ng/mL

3.1ng/mL

Sandwich

7.81-500ng/mL

Enzyme & Kinase;Endocrinology;Neuro science;

GLUD1; Glutamate dehydrogenase 1, mitochondrial

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Dehydrogenase 1 (GDH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Dehydrogenase 1 (GDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Dehydrogenase 1 (GDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Dehydrogenase 1 (GDH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.