Pig BMP4(Bone Morphogenetic Protein 4) ELISA Kit

 

Pig BMP4(Bone Morphogenetic Protein 4) ELISA Kit

Size

96T

Catalog no.

ELK5965

Price

738 EUR

Buy at gentaur.com
Assay length

3h

Tissue

bone

Standard

10ng/mL

Assay Type

Sandwich

Sensitivity

0.055ng/mL

Detection range

0.156-10ng/mL

Research Area

Developmental science;Bone metabolism;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Alternative Names

BMP2B; BMP2-B; BMP2B1; ZYME; DVR4; Bone morphogenetic protein 2B

About

Pigs and the smaller guinea pigs are frequent used as models for humans.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 4 (BMP4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 4 (BMP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 4 (BMP4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bone Morphogenetic Protein 4 (BMP4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.