Pig APOE(Apolipoprotein E) ELISA Kit

 

Pig APOE(Apolipoprotein E) ELISA Kit

Size

96T

Catalog no.

ELK5994

Price

738 EUR

Buy at gentaur.com
Assay length

3h

Standard

20ng/mL

Assay Type

Sandwich

Sensitivity

0.144ng/mL

Detection range

0.312-20ng/Ml

Research Area

Metabolic pathway;Cardiovascular biology;Hepatology;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Alternative Names

Apo-E; AD2; Apoprotein; Alzheimer Disease 2(E4-Associated,Late Onset

About

Pigs and the smaller guinea pigs are frequent used as models for humans.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein E (APOE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein E (APOE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein E (APOE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein E (APOE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.