Rat a1AT(Alpha-1-Antitrypsin) ELISA Kit

3h

500ng/mL

3.5ng/mL

Sandwich

7.81-500ng/mL

Rattus norvegicus

Enzyme & Kinase;Infection immunity;Hepatology;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

The a1AT(Alpha-1-Antitrypsin) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

SERPINA1; SPAAT; A1-AT; PI; Serpin Peptidase Inhibitor,Clade A(Alpha-1 Antiproteinase/AntiTrypsin)Member 1; Alpha-1 protease inhibitor; Short peptide from AAT; Serpin A1

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Antitrypsin (α1AT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-1-Antitrypsin (α1AT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Antitrypsin (α1AT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-Antitrypsin (α1AT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.