Rat GR(Glutathione Reductase) ELISA Kit

 

Rat GR(Glutathione Reductase) ELISA Kit

Size

96T

Catalog no.

ELK6025

Price

608 EUR

Buy at gentaur.com
Assay length

3h

Standard

10ng/mL

Assay Type

Sandwich

Sensitivity

0.064ng/mL

Detection range

0.156-10ng/mL

Latin name

Rattus norvegicus

Research Area

Metabolic pathway;

Alternative Names

GSR; GLUR; GRD1; Glutathione reductase, mitochondrial

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Reductase (GR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione Reductase (GR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione Reductase (GR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione Reductase (GR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.