2h
Pig GHRL(Ghrelin) ELISA Kit
Pig GHRL(Ghrelin) ELISA Kit
Assay length
Sensitivity
52.9pg/mL
Standard
10000pg/mL
Detection range
123.5-10000pg/mL
Assay Type
Competitive Inhibition
Alternative Names
MTLRP; Growth Hormone-Releasing Peptide
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About
Pigs and the smaller guinea pigs are frequent used as models for humans.
Research Area
Signal transduction;Metabolic pathway;Endocrinology;Gastroenterology;Hormone metabolism;
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Description
Ghrelin, GHRL gene, obestatin prepropeptide or lenomorelin from ghrelinergic cells in the gastrointestinal tract. GHRL is a neuropeptide in the central nervous system regulating appetite. Ghrelin is regulating the distribution and rate of use of body energy.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ghrelin (GHRL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ghrelin (GHRL) and unlabeled Ghrelin (GHRL) (Standards or samples) with the pre-coated antibody specific to Ghrelin (GHRL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ghrelin (GHRL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Ghrelin (GHRL) in the sample.