3h
Rat TPH1(Tryptophan Hydroxylase 1) ELISA Kit
Rat TPH1(Tryptophan Hydroxylase 1) ELISA Kit
Assay length
Standard
200ng/mL
Assay Type
Sandwich
Sensitivity
1.24ng/mL
Detection range
3.12-200ng/mL
Research Area
Enzyme & Kinase;
Latin name
Rattus norvegicus
Alternative Names
TPH; TPRH; TRPH; Tryptophan 5-Monooxygenase
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tryptophan Hydroxylase 1 (TPH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tryptophan Hydroxylase 1 (TPH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tryptophan Hydroxylase 1 (TPH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tryptophan Hydroxylase 1 (TPH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.