Pig VCAM1(Vascular Cell Adhesion Molecule 1) ELISA Kit

 

Pig VCAM1(Vascular Cell Adhesion Molecule 1) ELISA Kit

Size

96T

Catalog no.

ELK6133

Price

738 EUR

Buy at gentaur.com
Assay length

3h

Sensitivity

30pg/mL

Assay Type

Sandwich

Standard

5000pg/mL

Tissue

cell, vascular

Detection range

78.12-5000pg/mL

Alternative Names

CD106; INCAM-100; L1CAM

Research Area

CD & Adhesion molecule;Tumor immunity;Infection immunity;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

About

Pigs and the smaller guinea pigs are frequent used as models for humans.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Description

cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the VCAM1(Vascular Adhesion Molecule 1) ELISA Kit.For cells, cell lines and tissues in culture till half confluency.Whole adhesion and interacting molecules are  present in lysates used as reference for ELISA quantification of these molecules and their subunits.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Cell Adhesion Molecule 1 (VCAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vascular Cell Adhesion Molecule 1 (VCAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Cell Adhesion Molecule 1 (VCAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vascular Cell Adhesion Molecule 1 (VCAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.