Rat P4Ha1(Prolyl-4-Hydroxylase Alpha Polypeptide I) ELISA Kit

3h

10ng/mL

Sandwich

0.069ng/mL

0.156-10ng/mL

Enzyme & Kinase;

Rattus norvegicus

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Procollagen-Proline,2-Oxoglutarate 4-Dioxygenase(Proline 4-Hydroxylase),Alpha Polypeptide I

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

The P4Ha1(Prolyl-4-Hydroxylase Alpha Polypeptide I) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Hα1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Hα1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Hα1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Hα1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.