3h
96T
ELK6147
608 EUR
3h
10ng/mL
Sandwich
0.055ng/mL
0.156-10ng/mL
Rattus norvegicus
Infection immunity;
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Pantetheinase; Pantetheine hydrolase; Tiff66; Vascular non-inflammatory molecule 1
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vanin 1 (VNN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vanin 1 (VNN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vanin 1 (VNN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vanin 1 (VNN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.