Rat GC(Glucagon) ELISA Kit

2h

1500pg/mL

8.86pg/mL

Endocrinology;

18.52-1500pg/mL

Rattus norvegicus

Competitive Inhibition

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

GCG; GLP1; GLP2; GRPP; Glicentin-Related Polypeptide; Glucagen; Oxyntomodulin; Incretin hormone

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Glucagon gene and the glucagon like peptide or GLP1 gene are peptide hormones, produced by alpha cells of the pancreas to raise the concentration of glucose in the blood as opposite of insulin, which lowers the glucose. Anti human glucagon and glp1 antibodies are used to study diabetes.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Glucagon (GC) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Glucagon (GC) and unlabeled Glucagon (GC) (Standards or samples) with the pre-coated antibody specific to Glucagon (GC). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Glucagon (GC) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Glucagon (GC) in the sample.