Rat Thy1(Thy1 Cell Surface Antigen) ELISA Kit

3h

cell

10ng/mL

Sandwich

0.055ng/mL

0.156-10ng/mL

Rattus norvegicus

CD90; CDw90; THY mocyte differentiation antigen 1

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

CD & Adhesion molecule;Tumor immunity;Hematology;Neuro science;

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Antigens are peptides or recombinant or native dependent on the production method.For cells, cell lines and tissues in culture till half confluency.

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thy1 Cell Surface Antigen (Thy1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thy1 Cell Surface Antigen (Thy1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thy1 Cell Surface Antigen (Thy1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thy1 Cell Surface Antigen (Thy1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.