Rat KNG1(Kininogen 1) ELISA Kit

 

Rat KNG1(Kininogen 1) ELISA Kit

Size

96T

Catalog no.

ELK6413

Price

608 EUR

Buy at gentaur.com
Assay length

3h

Sensitivity

7.2pg/mL

Assay Type

Sandwich

Standard

1000pg/mL

Research Area

Hematology;

Detection range

15.6-1000pg/mL

Latin name

Rattus norvegicus

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Alternative Names

BDK; KNG; HMWK; Alpha-2-Thiol Proteinase Inhibitor; Bradykinin; Kallidin; Fitzgerald factor; High molecular weight kininogen; Williams-Fitzgerald-Flaujeac factor; Ile-Ser-Bradykinin

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kininogen 1 (KNG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kininogen 1 (KNG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kininogen 1 (KNG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kininogen 1 (KNG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.