Rat ANGPTL3(Angiopoietin Like Protein 3) ELISA Kit

3h

6.4pg/mL

Sandwich

1000pg/mL

15.62-1000pg/mL

Rattus norvegicus

Metabolic pathway;Tumor immunity;Hepatology;

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

ANGPT5; ANG-5; Angiopoietin 5; Angiopoietin-related protein 3

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiopoietin Like Protein 3 (ANGPTL3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiopoietin Like Protein 3 (ANGPTL3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiopoietin Like Protein 3 (ANGPTL3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Angiopoietin Like Protein 3 (ANGPTL3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.