Rat COL3a1(Collagen Type III Alpha 1) ELISA Kit

 

Rat COL3a1(Collagen Type III Alpha 1) ELISA Kit

Size

96T

Catalog no.

ELK6514

Price

608 EUR

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Assay length

3h

Standard

40ng/mL

Assay Type

Sandwich

Sensitivity

0.277ng/mL

Detection range

0.625-40ng/mL

Latin name

Rattus norvegicus

Research Area

Metabolic pathway;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Gene

Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )

Description

The COL3a1(Collagen III Alpha 1) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Alternative Names

COL3-A1; COL3A1; EDS4A; Collagen Type III Alpha 1; Ehlers-Danlos Syndrome Type IV Autosomal Dominant; Collagen Alpha-1(III)chain

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type III Alpha 1 (COL3α1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type III Alpha 1 (COL3α1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type III Alpha 1 (COL3α1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type III Alpha 1 (COL3α1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.