Rat CLDN4(Claudin 4) ELISA Kit

 

Rat CLDN4(Claudin 4) ELISA Kit

Size

96T

Catalog no.

ELK6579

Price

608 EUR

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Assay length

3h

Standard

10ng/mL

Assay Type

Sandwich

Sensitivity

0.060ng/mL

Detection range

0.156-10ng/mL

Latin name

Rattus norvegicus

Research Area

Signal transduction;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Alternative Names

CPE-R; CPER; CPETR; CPETR1; WBSCR8; hCPE-R; Clostridium Perfringens Enterotoxin Receptor 1; Williams-Beuren Syndrome Chromosomal Region 8 Protein

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 4 (CLDN4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Claudin 4 (CLDN4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 4 (CLDN4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Claudin 4 (CLDN4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.