Rat CHRM2(Cholinergic Receptor, Muscarinic 2) ELISA Kit

 

Rat CHRM2(Cholinergic Receptor, Muscarinic 2) ELISA Kit

Size

96T

Catalog no.

ELK6596

Price

608 EUR

Buy at gentaur.com
Assay length

3h

Standard

10ng/mL

Assay Type

Sandwich

Sensitivity

0.053ng/mL

Detection range

0.156-10ng/mL

Latin name

Rattus norvegicus

Research Area

Signal transduction;Cardiovascular biology;Neuro science;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Alternative Names

CHR-M2; HM2; M-AChRM2; Muscarinic Acetylcholine Receptor M2; Cholinergic Receptor,Muscarinic 2

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Description

The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Muscarinic 2 (CHRM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholinergic Receptor, Muscarinic 2 (CHRM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholinergic Receptor, Muscarinic 2 (CHRM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholinergic Receptor, Muscarinic 2 (CHRM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.