3h
Rat ELA2A(Elastase 2A) ELISA Kit
Rat ELA2A(Elastase 2A) ELISA Kit
Assay length
Assay Type
Sandwich
Standard
4000pg/mL
Sensitivity
22.5pg/mL
Detection range
62.50-4000pg/mL
Research Area
Enzyme & Kinase;
Latin name
Rattus norvegicus
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Alternative Names
ELA1; PE-1; CELA2A; Chymotrypsin-Like Elastase Family Member 2A
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastase 2A (ELA2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elastase 2A (ELA2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastase 2A (ELA2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Elastase 2A (ELA2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.