Rat BRAK(Breast And Kidney Expressed Chemokine) ELISA Kit

 

Rat BRAK(Breast And Kidney Expressed Chemokine) ELISA Kit

Size

96T

Catalog no.

ELK6663

Price

608 EUR

Buy at gentaur.com
Assay length

3h

Sensitivity

6.2pg/mL

Assay Type

Sandwich

Standard

1000pg/mL

Tissue

breast, kidney

Detection range

15.6-1000pg/mL

Latin name

Rattus norvegicus

Research Area

Cytokine;Infection immunity;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Description

Chemokines, chemokine receptors, ligands , motif chemokines and cytokines are supplied by ELK Biotech in 1.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Alternative Names

CXCL14; SCYB14; BRAK; NJAC; Bolekine; Kec; MIP2-G; MIP2g; BMAC; KS1; Chemokine(C-X-C-Motif)ligand 14; Small Inducible Cytokine Subfamily B(Cys-X-Cys)Member 14

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Breast And Kidney Expressed Chemokine (BRAK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Breast And Kidney Expressed Chemokine (BRAK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Breast And Kidney Expressed Chemokine (BRAK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Breast And Kidney Expressed Chemokine (BRAK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.