Cattle RNASE1(Ribonuclease A) ELISA Kit

 

Cattle RNASE1(Ribonuclease A) ELISA Kit

Size

96T

Catalog no.

ELK6668

Price

738 EUR

Buy at gentaur.com
Assay length

3h

Standard

200ng/mL

Assay Type

Sandwich

Sensitivity

1.31ng/mL

Detection range

3.12-200ng/mL

Research Area

Enzyme & Kinase;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Alternative Names

RNASE; Rnase-A; RNase UpI-1; RIB-1; Ribonuclease,RNase A Family,1(Pancreatic)

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease A (RNASE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease A (RNASE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease A (RNASE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribonuclease A (RNASE1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.