Rat PKBb(Protein Kinase B Beta) ELISA Kit

3h

20ng/mL

Sandwich

0.115ng/mL

0.312-20ng/mL

Rattus norvegicus

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Enzyme & Kinase;Metabolic pathway;Endocrinology;Hormone metabolism;

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

AKT2; PKBBETA; PRKB-B; RAC-BETA; RAC-Beta Serine/Threonine-Protein Kinase; V-Akt Murine Thymoma Viral Oncogene Homolog 2

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase B Beta (PKBβ). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase B Beta (PKBβ). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase B Beta (PKBβ), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase B Beta (PKBβ) in the samples is then determined by comparing the O.D. of the samples to the standard curve.