3h
Rat FOXP3(Forkhead Box Protein P3) ELISA Kit
Rat FOXP3(Forkhead Box Protein P3) ELISA Kit
Assay length
Standard
20ng/mL
Assay Type
Sandwich
Sensitivity
0.113ng/mL
Detection range
0.312-20ng/mL
Latin name
Rattus norvegicus
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Research Area
Signal transduction;Apoptosis;Tumor immunity;Infection immunity;Immune molecule;
Alternative Names
FOX-P3; AIID; DIETER; IPEX; JM2; PIDX; XPID; SCURFIN; Immune Dysregulation,Polyendocrinopathy,Enteropathy,X-Linked
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Gene
Forkhead Box Protein P3 scurfin FOXP3, is a protein involved in immune system responses. A member of the FOX protein family, FOXP3 appears to function as a master regulator of the regulatory pathway in the development and function of regulatory T cells. Foxp3 or forkhead box P3 or skurfin or scurfin or skurfina is also known as IPEX, AIID, PIDX, XPID and DIETER.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Forkhead Box Protein P3 (FOXP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Forkhead Box Protein P3 (FOXP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Forkhead Box Protein P3 (FOXP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Forkhead Box Protein P3 (FOXP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.