3h
Rat LFA1a(Lymphocyte Function Associated Antigen 1 Alpha) ELISA Kit
Rat LFA1a(Lymphocyte Function Associated Antigen 1 Alpha) ELISA Kit
Assay length
Standard
10ng/mL
Assay Type
Sandwich
Tissue
lymphocyte
Sensitivity
0.055ng/mL
Detection range
0.156-10ng/mL
Latin name
Rattus norvegicus
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Research Area
Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Alternative Names
CD11a; ITG-AL; LFA-1; LFA1-A; ITGAL; Integrin,Alpha L; CD11 antigen-like family member A; Leukocyte function-associated molecule 1 alpha chain
Description
The LFA1a(Lymphocyte Associated Antigen 1 Alpha) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.Antigens are peptides or recombinant or native dependent on the production method.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphocyte Function Associated Antigen 1 Alpha (LFA1α). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lymphocyte Function Associated Antigen 1 Alpha (LFA1α). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphocyte Function Associated Antigen 1 Alpha (LFA1α), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lymphocyte Function Associated Antigen 1 Alpha (LFA1α) in the samples is then determined by comparing the O.D. of the samples to the standard curve.