Human NFATC2(Nuclear Factor Of Activated T-Cells, Cytoplasmic 2) ELISA Kit

 

Human NFATC2(Nuclear Factor Of Activated T-Cells, Cytoplasmic 2) ELISA Kit

Size

96T

Catalog no.

ELK6857

Price

608 EUR

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Assay length

3h

Tissue

cells

Sensitivity

5.9pg/mL

Assay Type

Sandwich

Standard

1000pg/mL

Detection range

15.62-1000pg/mL

Research Area

Infection immunity;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Alternative Names

NFAT1; NFATP; NF-ATP; NFAT pre-existing subunit; T-cell transcription factor NFAT1

Description

Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.