Rat GHRL(Ghrelin) ELISA Kit

2h

2000pg/mL

10.18pg/mL

Endocrinology;

24.69-2000pg/mL

Rattus norvegicus

Competitive Inhibition

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

MTLRP; Preproghrelin; Growth Hormone Secretagogue Receptor Ligand; Appetite-regulating hormone; Growth hormone-releasing peptide; Motilin-related peptide

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Ghrelin, GHRL gene, obestatin prepropeptide or lenomorelin from ghrelinergic cells in the gastrointestinal tract. GHRL is a neuropeptide in the central nervous system regulating appetite. Ghrelin is regulating the distribution and rate of use of body energy.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ghrelin/Obestatin Preprohormone (GHRL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ghrelin/Obestatin Preprohormone (GHRL) and unlabeled Ghrelin/Obestatin Preprohormone (GHRL) (Standards or samples) with the pre-coated antibody specific to Ghrelin/Obestatin Preprohormone (GHRL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ghrelin/Obestatin Preprohormone (GHRL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Ghrelin/Obestatin Preprohormone (GHRL) in the sample.