3h
Cattle INHB(Inhibin B) ELISA Kit
Cattle INHB(Inhibin B) ELISA Kit
Assay length
Alternative Names
INH-B
Assay Type
Sandwich
Standard
2000pg/mL
Sensitivity
11.5pg/mL
Detection range
31.25-2000pg/mL
Research Area
Reproductive science;Genetic science;Hormone metabolism;
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inhibin B (INHB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inhibin B (INHB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inhibin B (INHB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inhibin B (INHB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.